"We need the co-operation of each and every citizen of this province and country to bring the perpetrators to book and stop this slaughter from continuing," Limpopo provincial commissioner Lieutenant General Simon Mpende said on Sunday.
Mpende said the police could not fight the battle alone and public assistance was needed.
In 2011, 74 rhino were killed for their horns in Limpopo. In January, at least 10 rhino were poached in the province. More than 165 rhino have been killed since 2008. Thirty-eight suspected poachers are awaiting trial.