England selectors have picked 13 cricketers to face South Africa in a four-day match in Canterbury, starting on 9 August.
The match will serve as the tourists' warm-up game in advance of first Test against England at Lord's from 17 August.
The England Lions, effectively England 'A', will be coached by Paul Collingwood, who is an assistant coach to the England national side.
The squad includes SA-born former England Test opener Keaton Jennings, who is the son of former Proteas coach Ray Jennings.
Jennings, 30, played 17 Tests for England between 2016 and 2019 but averaged just 25.19 with the bat.
Sam Billings (Kent, captain), Harry Brook (Yorkshire), Sam Conners (Derbyshire), Sam Cook (Essex), Ben Duckett (Nottinghamshire), Will Jacks (Surrey), Keaton Jennings (Lancashire), Dan Lawrence (Essex), Craig Overton (Somerset), Liam Patterson-White (Nottinghamshire), James Rew (Somerset), Ollie Robinson (Sussex), Dom Sibley (Warwickshire)
Mo Bobat, the ECB's performance director, said in a statement: "We are delighted that the Lions will have a competitive fixture against the South African tourists. The squad strongly represents some of the country's current, next best and future England players. For some players, this game will be important preparation going into the Test series.
Bobat added: "These matches for the Lions are pivotal as we try to build the depth of players that can further enhance the way the Test team have been playing this summer. It is a reward for players who have scored runs and taken wickets this season in the County Championship campaign and have caught our eye in how they've gone about their business.
"I would also like to thank The Hundred teams and respective Head Coaches who have enabled the eight players who are playing in the competition the opportunity to play for the Lions. We are grateful for their support."