Madrid - Chris Froome's hopes of winning the Tour of Catalonia are almost certainly over after the Briton suffered a crash during Tuesday's second stage, which was won by Australian Michael Matthews.
With less than 40km to go, four-time Tour de France champion Froome fell along with Bora-Hansgrohe pair Andres Schillinger and Jay McCarthy before finishing more than 13 minutes behind Matthews of Team Sunweb.
Froome, who is racing for the first time since his season debut in January, was left with a nasty gash down the top of his right leg and the Team Sky rider will surely have to concede defeat in what had been dubbed a showdown with Movistar duo Alejandro Valverde and Nairo Quintana.
At the end of a 167km-stage from Mataro to Saint Feliu de Guixoles, Lotto Soudal's Thomas de Gendt remained in the overall lead following his victory in the first stage on Monday and even extended his advantage after a succesful attack on the final climb.
De Gendt sits 2:47 ahead of Valverde in the general classification, with Matthews a further second behind in third.
The Belgian was the first to emerge over the Alt de Romanya before Valverde and Quintana seized the initiative in the sprint with just under 8km left.
Maximilian Schachmann and Dion Smith both made late challenges but it was Matthews that pipped Valverde to the line, with Mitchelton-Scott's Darryl Impey coming in third.
"It was really hard but I was pretty confident in an uphill sprint like this," Matthews said. "
"After my bad luck this year it's just incredible. I'm so happy.
"When I saw Valverde on my wheel, struggling in the sprint, I knew I was going fast and had some good power, so I'm so thankful to my team for believing in me for this long and I came up with a nice victory."
Wednesday's 179km third stage heads north-west into the mountains finishing in the resort of Valter at 2 000m altitude in the Pyrenees.
1. Thomas De Gendt (BEL/LOT) 8hrs 23min 50sec, 2. Alejandro Valverde (ESP/MOV) at 2min 47sec, 3. Michael Matthews (AUS/SUN) 2:48, 4. Maximilian Schachmann (GER/BOR) same time, 5. Daryl Impey (RSA/MIT) 2:54, 6. Nairo Quintana (COL/MOV) 2:56, 7. Egan Bernal (COL/SKY) 2:57, 8. Steven Kruijswijk (NED/JUM) s.t., 9. Luis Angel Mate Mardones (ESP/COF) s.t., 10. Patrick Bevin (NZL/CCC) 2:58.
1. Michael Matthews (AUS/SUN) 167km in 4hrs 9mins 34sec, 2. Alejandro Valverde (ESP/MOV) s.t., 3. Daryl Impey (RSA/MIT) s.t., 4. Maximilian Schachmann (GER/BOR) s.t., 5. Odd Christian Eiking (NOR/WGG) s.t., 6. James Knox (GBR/DQT) s.t., 7. Patrick Bevin (NZL/CCC) s.t., 8. Enrico Gasparotto (ITA/TDD), 9. Davide Formolo s.t., (ITA/BOH) 10. Daniel Martin (IRL/UAD) s.t.