The Lions have picked a young side to tackle the EP Elephants at the Nelson Mandela Bay Stadium on Wednesday afternoon.
The team shows several changes to the one which beat the Pumas 32-28 In Johannesburg in their preparation series opener.
The team is brimming with players who caught the eye during the national Under-21 tournament which was held at Ellis Park last year and coaches Mzwikhe Nkosi and Wessel Roux have full confidence in their charges.
"The changes are therefore not due to any players being demoted, but rather another opportunity to scrutinise and test everyone," the Lions said in a statement.
Kick-off is at 16:45 on Wednesday.
15 Courtney Winnaar (captain), 14 Qeutyne Oerson, 13 Athi Mayinje, 12 Sherwin Slater, 11 Christopher Hollis, 10 Karlo Aspeling, 9 Josh Alldermann, 8 Zingisa April, 7 Anele Lungisa, 6 CJ Velleman, 5 Arnold Kleyn, 4 Athenkosi Khetani, 3 Emile Klaassen, 2 Sithembiso Befile, 1 Siya Nzuzo.
Substitutes: 16 Robin Stevens, 17 Lyle Lombard, 18 Lupumlo Mgcuca, 19 Mncedisi Dlwengu, 20 Bevin Prinsloo, 21 Diego Williams, 22 Wian van der Sandt, 23 Riaan Arends
15 Divan Rossouw, 14 Prince Nkabinde, 13 Manuel Rass, 12 James Mollentze, 11 Ngia Selengbe, 10 Tiaan Swanepoel, 9 Dillon Smit, 8 Ruhan Straeuli, 7 Sibusiso Sangweni, 6 Mark Snyman, 5 Reinhard Nothnagel (captain), 4 Ruben Schoeman, 3 Wiehahn Herbst, 2 Dameon Venter, 1 Nathan McBeth
Substitutes: 16 Morne Brandon, 17 Banele Mthenjane, 18 Asenathi Nthlabakanye, 19 Mandisi Mtiyane, 20 Izan Esterhuizen, 21 Morne van den Berg, 22 Jordan Hendrikse, 23 Luke Rossouw, 24 Sibusiso Shongwe, 25 Jarod Cairns