- The Sunwolves have reached the end of the road in Super Rugby as their bid to join Australia's domstic event faltered.
- The Japanese outfit will also no be involved in next year's proposed 14-team Super Rugby event.
- Australia's new series will feature the Reds, Waratahs, Brumbies, Melbourne Rebels and Western Force - and will kick off on 3 July.
Japan's Sunwolves bowed out of Super Rugby on Monday as they failed to join an Australian offshoot of the coronavirus-hit tournament.
The Sunwolves, who are being kicked out of Super Rugby at the end of the season, said Australian officials had told them they won't be able to take part.
The series between Australia's four Super Rugby teams - Reds, Waratahs, Brumbies and Melbourne Rebels - along with the previously axed Western Force, is set to kick off on 3 July.
Super Rugby, which also includes teams from New Zealand, South Africa and Argentina, has been on hold since March.
"We are announcing that we have been told by the Australian rugby association officially that we are unable to participate in the competition," a Sunwolves statement said.
The decision ends a nearly five-year run in Super Rugby for the Sunwolves, who won only nine games in that time and were on the receiving end of some giant scorelines.
The Tokyo-based side would have had to quarantine for 14 days on arrival in Australia, and they also faced problems in securing a permanent base for 12 weeks.
They said they had consulted with Australia's rugby authorities and government, but "we reached the conclusion that we will not be ready to join matches in Australia from 3 July".
"We apologise to people who had been looking forward to the resumption of matches," said Sunwolves CEO Yuji Watase.
"Following this decision, the Sunwolves' time with Super Rugby, which has seen us participate for the past five seasons, will end.
"We are bitterly disappointed to conclude the season in this way, but we also feel honoured to have participated in the best rugby competition."
The Sunwolves, who also played home matches in Singapore and Hong Kong, were introduced in 2016 to bring rugby to new markets.
But southern hemisphere governing body Sanzaar (South Africa, New Zealand, Australia and Argentina Rugby) was reluctant to bankroll the perennial wooden-spooners after Japan's rugby board withdrew financial support.
Rugby Australia's interim chief executive, Rob Clarke, said officials had been trying to work through "significant challenges" to field a Sunwolves team in Australia.
"However, the reality is, given the time frame available the team's preparation for the competition would be severely compromised," he said in a statement.
"All parties agree that despite our collective efforts and desire to see the Sunwolves take part in the competition, under the current circumstances their participation will not be feasible."
The Sunwolves' exit comes despite the success of last year's Rugby World Cup in Japan and the lure of Japan's well-heeled Top League, which has attracted a series of marquee players from overseas.
New Zealand, like Australia, is also planning a domestic competition, kicking off on June 13, to fill the hole left by Super Rugby's postponement.