Serena Williams tasted bitter defeat on her return to singles tennis at Wimbledon on Tuesday as Rafael Nadal overcame a huge scare to progress to the second round.
Elsewhere on day two of the Championships, title contender Matteo Berrettini was forced to pull out with coronavirus while women's top seed Iga Swiatek extended her winning streak to 36 matches.
Seven-time champion Williams went down to unseeded Harmony Tan of France 7-5, 1-6, 7-6 (10/7) in her first singles encounter since an injury forced her out of her first-round match at Wimbledon last year.
Williams, 40, won the last of her Wimbledon singles titles six years ago but reached the final in 2018 and 2019.
"I'm so emotional now," said Tan. "She's a superstar. When I was young, I was watching her so many times on the TV.
"For my first Wimbledon it's wow - just wow.
"When I saw the draw, I was really scared. She's such a legend. I thought if I can win one game, two games, that would be really good for me."
Williams was far below her imperious best on Tuesday, struggling from the start with her form and fitness.
The 23-time Grand Slam singles champion, cheered by the crowd as she made her entrance, lost the first set but broke early in the second set and went on to level the match.
But she faltered while serving for the match and faded badly in the third-set tie-break after taking a 4-0 lead.
She finished with 61 winners but 54 unforced errors the 3-hour, 11-minute evening match.
Earlier, an off-key Nadal, already halfway to a calendar Grand Slam after winning the Australian Open and French Open, dug deep to beat Francisco Cerundolo 6-4, 6-3, 3-6, 6-4.
The two-time Wimbledon champion, who has not played at the tournament since 2019, thanked the crowd for their wholehearted support.
"It's not a surface that we play very often, and especially in my case, for different reasons, the past three years I didn't put any foot on a grass court, so it always takes a while," said Nadal, 36.
"It was my first match and as I know, every day is a test and today was one of these important tests."
The Spaniard appeared to be coasting to victory when he took a two-set lead but his Argentine opponent found a new level, winning the third set and going a break up early in the fourth.
But from 4-2 down the second seed found an extra gear, winning the next four games to seal the match in a little over three and a half hours, roared on by the Centre Court crowd.
The 22-time Grand Slam champion played the entire French Open with his troublesome left foot anaesthetised but he has received treatment since then and was moving well on Tuesday.
Canada's Felix Auger-Aliassime, who took Nadal to five sets at the French Open, crashed out of the tournament at the hands of American Maxime Cressy.
The sixth seed lost 6-7 (5/7), 6-4, 7-6 (11/9), 7-6 (7/5), removing another potential obstacle in the path of Nadal, who next faces Lithuania's Ricardas Berankis.
Men's fourth seed Stefanos Tsitsipas beat Swiss qualifier Alexander Ritschard in four sets.
The early action at the All England Club was overshadowed by the announcement from Berrettini - last year's runner-up - that coronavirus had forced him out of the tournament.
"I am heartbroken to announce that I need to withdraw from @wimbledon due to a positive Covid-19 test result," the 26-year-old Italian wrote on Instagram.
Berrettini was widely regarded as one of the biggest threats to defending champion Novak Djokovic at Wimbledon, having captured back-to-back grass-court titles in Stuttgart and Queen's.
He is the second man to withdraw from Wimbledon with Covid in two days after 2017 runner-up Marin Cilic of Croatia also tested positive.
The Italian was a potential semi-final opponent for Nadal.
It is business as usual at the Grand Slam this year after the tournament was cancelled due to Covid in 2020 and last year was played in front of reduced crowds.
The All England Club said in a statement that protocols remained in place to minimise the risk of infection.
Poland's world number one Swiatek, who has won her past six tournaments - coasted into the second round with a routine 6-0, 6-3 victory against Croatian qualifier Jana Fett.
There were also straightforward wins in the women's draw for 2019 champion Simona Halep and fourth seed Paula Badosa.
Beaten French Open finalist Coco Gauff, the 11th seed, recovered from losing the first set against Romania's Elena-Gabriela Ruse to win 2-6, 6-3, 7-5.
Wimbledon results on Tuesday on the second day of the 2022 championships at the All England Club (x denotes seeded player):
Reilly Opelka (USA x15) bt Carlos Taberner (ESP) 7-6 (7/5), 6-4, 6-4
Mikael Ymer (SWE) bt Daniel Altmaier (GER) 6-3, 7-5, 7-5
Christian Harrison (USA) bt Jay Clarke (GBR) 7-6 (7/3), 6-1, 7-6 (8/6)
David Goffin (BEL) bt Radu Albot (MDA) 6-2, 6-2, 7-6 (7/5)
Sebastian Baez (ARG x31) bt Taro Daniel (JPN) 6-4, 6-4, 7-5
Ryan Peniston (GBR) bt Henri Laaksonen (SUI) 6-4, 6-3, 6-2
Steve Johnson (USA) bt Grigor Dimitrov (BUL x18) 4-6, 5-2 - retired
Christian Garín (CHI) bt Elias Ymer (SWE) 6-3, 7-5, 6-4
Hugo Grenier (FRA) bt Marc-Andrea Huesler (SUI) 6-3, 7-6 (7/0), 6-7 (5/7), 2-6, 6-4
Benjamin Bonzi (FRA) bt Zdenek Kolár (CZE) 6-3, 6-3, 6-1
Jenson Brooksby (USA x29) bt Mikhail Kukushkin (KAZ) 6-3, 6-2, 6-3
Alex De Minaur (AUS x19) bt Hugo Dellien (BOL) 6-1, 6-3, 7-5
Jack Draper (GBR) bt Zizou Bergs (BEL) 6-4, 6-4, 7-6 (7/4)
Liam Broady (GBR) bt Lukas Klein (SVK) 4-6, 6-3, 7-5, 6-7 (2/7), 6-3
Diego Schwartzman (ARG x12) bt Stefan Kozlov (USA) 6-3, 6-2, 6-2
Denis Shapovalov (CAN x13) bt Arthur Rinderknech (FRA) 6-1, 6-7 (6/8), 6-7 (4/7), 6-4, 6-1
Brandon Nakashima (USA) bt Nicola Kuhn (ESP) 6-3, 6-7 (1/7), 6-3, 6-2
Daniel Galán (COL) bt Dominik Koepfer (GER) 6-4, 7-5, 7-6 (7/1)
Roberto Bautista Agut (ESP x17) bt Attila Balazs (HUN) 6-1, 6-0, 6-3
Filip Krajinovic (SRB x26) bt Jiri Lehecka (CZE) 5-7, 6-4, 6-7 (5/7), 7-6 (7/5), 6-4
Nick Kyrgios (AUS) bt Paul Jubb (GBR) 3-6, 6-1, 7-5, 6-7 (3/7), 7-5
Jordan Thompson (AUS) bt Roberto Carballés Baena (ESP) 6-4, 6-1, 6-1
Stefanos Tsitsipas (GRE x4) bt Alexander Ritschard (SUI) 7-6 (7/1), 6-3, 5-7, 6-4
Maxime Cressy (USA) bt Félix Auger-Aliassime (CAN x6) 6-7 (5/7), 6-4, 7-6 (11/9), 7-6 (7/5)
Jack Sock (USA) bt Bernabe Zapata Miralles (ESP) 7-6 (8/6), 6-4, 6-4
Dennis Novak (AUT) bt Facundo Bagnis (ARG) 7-6 (9/7), 2-6, 6-4, 6-3
Jason Kubler (AUS) bt Dan Evans (GBR x28) 6-1, 6-4, 6-3
Marcos Giron (USA) bt Holger Rune (DEN x24) 6-3, 7-5, 6-4
Alex Molcan (SVK) bt Pedro Martínez (ESP) 4-6, 6-4, 6-4, 6-1
Alastair Gray (GBR) bt Chun Hsin Tseng (TAI) 6-3, 6-3, 7-6 (7/3)
Taylor Fritz (USA x11) bt Lorenzo Musetti (ITA) 6-4, 6-4, 6-3
Mackenzie McDonald (USA) bt Nuno Borges (POR) 6-4, 6-4, 7-6 (7/3)
Richard Gasquet (FRA) bt João Sousa (POR) 7-6 (9/7), 6-2, 4-6, 4-6, 6-3
Emil Ruusuvuori (FIN) bt Yoshihito Nishioka (JPN) 6-2, 6-2, 4-6, 6-4
Botic van de Zandschulp (NED x21) bt Feliciano Lopez (ESP) 6-2, 6-3, 6-3
Lorenzo Sonego (ITA x27) bt Denis Kudla (USA) 6-7 (6/8), 6-3, 7-5, 4-6, 6-2
Hugo Gaston (FRA) bt Alexei Popyrin (AUS) 6-2, 4-6, 0-6, 7-6 (7/3), 6-3
Ricardas Berankis (LTU) bt Sam Querrey (USA) 6-4, 7-5, 6-3
Rafael Nadal (ESP x2) bt Francisco Cerundolo (ARG) 6-4, 6-3, 3-6, 6-4
Iga Swiatek (POL x1) bt Jana Fett (CRO) 6-0, 6-3
Lesley Pattinama Kerkhove (NED) bt Sonay Kartal (GBR) 6-4, 3-6, 6-1
Claire Liu (USA) bt Nuria Parrizas Diaz (ESP) 7-5, 6-3
Alize Cornet (FRA) bt Yulia Putintseva (KAZ x27) 6-3, 7-6 (7/5)
Ajla Tomljanovic (AUS) bt Jil Teichmann (SUI x18) 6-2, 6-3
Catherine Harrison (USA) bt Arantxa Rus (NED) 6-1, 6-4
Viktorija Golubic (SUI) bt Andrea Petkovic (GER) 6-4, 6-3
Barbora Krejcikova (CZE x13) bt Maryna Zanevska (BEL) 7-6 (7/4), 6-3
Zheng Qinwen (CHN) bt Sloane Stephens (USA) 7-6 (7/1), 7-5
Bianca Andreescu (CAN) bt Emina Bektas (USA) 6-1, 6-3
Elena Rybakina (KAZ x17) bt Coco Vandeweghe (USA) 7-6 (7/2), 7-5
Petra Martic (CRO) bt Shelby Rogers (USA x30) 6-2, 7-6 (7/5)
Kristína Kucová (SVK) bt Laura Pigossi (BRA) 7-5, 6-0
Paula Badosa (ESP x4) bt Louisa Chirico (USA) 6-2, 6-1
Irina Maria Bara (ROM) bt Chloe Paquet (FRA) 6-2, 6-4
Ana Bogdan (ROM) bt Dayana Yastremska (UKR) 6-2, 6-2
Petra Kvitova (CZE x25) bt Jasmine Paolini (ITA) 2-6, 6-4, 6-2
Magdalena Frech (POL) bt Camila Giorgi (ITA x21) 7-6 (7/4), 6-1
Anna Karolína Schmiedlova (SVK) bt Rebecca Peterson (SWE) 7-5, 0-6, 6-3
Kirsten Flipkens (BEL) bt Jaimee Fourlis (AUS) 7-5, 6-2
Simona Halep (ROM x16) bt Karolína Muchová (CZE) 6-3, 6-2
Coco Gauff (USA x11) bt Gabriela Ruse (ROM) 2-6, 6-3, 7-5
Mihaela Buzarnescu (ROM) bt Nastasja Schunk (GER) 6-4, 6-2
Lauren Davis (USA) bt Madison Brengle (USA) 6-2, 7-5
Amanda Anisimova (USA x20) bt Yuan Yue (CHN) 6-3, 6-4
Sara Sorribes Tormo (ESP x32) bt Christina McHale (USA) 6-2, 6-1
Harmony Tan (FRA) bt Serena Williams (USA) 7-5, 1-6, 7-6 (10/7)
Katie Boulter (GBR) bt Clara Burel (FRA) 7-5, 6-3
Zhang Shuai (CHN x33) bt Misaki Doi (JPN) 6-4, 6-0
Marta Kostyuk (UKR) bt Katie Swan (GBR) 4-6, 6-4, 6-4
Maria Sakkari (GRE x5) bt Zoe Hives (AUS) 6-1, 6-4
Viktoriya Tomova (BUL) bt Daria Saville (AUS) 7-5, 3-6, 7-5
Tatjana Maria (GER) bt Astra Sharma (AUS) 4-6, 6-3, 6-4
Yanina Wickmayer (BEL) bt Zhu Lin (CHN) 6-4, 6-2
Jelena Ostapenko (LAT x12) bt Oceane Dodin (FRA) 6-4, 6-4
Wang Qiang (CHN) bt Belinda Bencic (SUI x14) 6-4, 5-7, 6-2
Heather Watson (GBR) bt Tamara Korpatsch (GER) 6-7 (7/9), 7-5, 6-2