A UN employee from Liberia who was transported to the eastern German city of Leipzig after contracting Ebola died overnight into Tuesday, the hospital said.
The 56-year-old man had been transported to St Georg hospital in Leipzig on October 9 for treatment.
He had arrived in a modified Gulfstream jet equipped with an isolation chamber, and was met by a medical team in biohazard suits and two ambulances.
The hospital has six rooms in its tropical medicine clinic. Hospital authorities said there is no risk of further infection.
Another Ebola patient, from Uganda, remains in a hospital in Frankfurt, and a third, from Senegal, was released in good health from a Hamburg clinic after five weeks' treatment.
The World Health Organization (WHO) estimates the death toll resulting from the worst outbreak of Ebola on record to have reached 4033.
In Liberia, the hardest-hit West African country, 2316 people have died. The death toll in Sierra Leone and Guinea stands at nearly 1000 respectively, according to the latest WHO update.
- Birgit Zimmermann and Nico Esch