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The divas first fell out when critics compared Gaga's Born This Way to Madonna's Express Yourself and the pop icon accused the younger star of ripping off her tune.
Last year, Madonna said, "The only time I ever criticised Lady Gaga was when I felt like she blatantly ripped off one of my songs. It's got nothing to do with 'she's taking my crown' or 'she's in some space of mine'. She has her thing. I do think she's a very talented singer and songwriter. It was just that one issue."
Read more: Lady Gaga doesn’t care what Madonna thinks
Although the feud simmered down for a while, the war of words appears to have been reignited by Lady Gaga after the pop sensation took a subtle swipe at Madonna in a new Beats 1 Radio chat with Zane Lowe.
"Madonna and I are very different," the 30-year-old told Lowe on Thursday when asked about links between the two singers. "I wouldn't make that comparison at all and I don't mean to disrespect Madonna; she's a nice lady and she's had a fantastic huge career, biggest pop star of all time.
Read more: Lady Gaga for American Horror Story
"But I play a lot of instruments. I write all my own music. I spend hours and hours a day in the studio. I'm a producer. I'm a writer. What I do is different.
"I'm not just rehearsing over and over again to put on a show there is a spontaneity in my work. I allow myself to fail. I allow myself to break. I'm not afraid of my flaws... There are major differences between me and her and I don't mean that in a disrespectful way. I just will not be compared to anyone anymore. I am who the f**k I am and this is me."
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